Cell-Free Assay of G-Protein-Coupled Receptors Using Fluorescence Polarization
نویسندگان
چکیده
منابع مشابه
Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay
For more than 20 years, reverse pharmacology has been the preeminent strategy to discover the activating ligands of orphan G protein-coupled receptors (GPCRs). The onset of a reverse pharmacology assay is the cloning and subsequent transfection of a GPCR of interest in a cellular expression system. The heterologous expressed receptor is then challenged with a compound library of candidate ligan...
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ژورنال
عنوان ژورنال: Journal of Biomolecular Screening
سال: 2008
ISSN: 1087-0571,1552-454X
DOI: 10.1177/1087057108318332